Plant Extract as Green Synthesis Agent for Silver Nanoparticle Synthesis and Evaluation of Their Antibacterial Activity
Abstract
Silver nanoparticles (AgNPs) have gained significant attention due to their eco-friendly synthesis and remarkable antibacterial properties. In this study, AgNPs were synthesized with a silver nitrate solution and bio-reducing agents extracted from neem (Azadirachta indica) leaves through a simple and cost-effective water bath treatment method. The synthesized nanoparticles were characterized using UV-Visible spectroscopy and X-ray diffraction (XRD) analysis. The antibacterial activity of the AgNPs was evaluated against Staphylococcus aureus using standard antimicrobial assays. The results demonstrated significant antibacterial efficiency. This eco-friendly approach provides a sustainable and green alternative for nanoparticle synthesis, reducing the hazardous environmental impact associated with conventional chemical methods
Keywords: Anti bacterial study, Plant extract, Silver Nano Particles
References
- 1) Pandian, H., et al. (2023). Azadirachta indica leaf extract mediated silver nanoparticles impregnated nanocomposite film (AgNP/MCC/starch/whey protein) for food packaging applications. Environmental Research, 216(2), 114641.
- 2) Mudenda, S., Banda, M., Mohamed, S., & Chabalenge, B. (2023). Phytochemical composition and antibacterial activities of Azadirachta indica (neem): Significance of traditional medicine in combating infectious diseases and antimicrobial resistance. Journal of Pharmacognosy and Phytochemistry, 12(5), 256–263.
- 3) Sangeetha, V., & Ponnuswami, V. (2014). Synthesis and characterization of silver nanoparticles using Moringa oleifera pod extract. Biosciences, 7(8), 675–679.
- 4) Peter, L., Sivagnanam, S., & Jayanthi, A. (2015). Synthesis of silver nanoparticles using plant extracts and analysis of their antimicrobial property. Journal of Saudi Chemical Society, 19(3), 311–317.
- 5) Shankar, S. S., Ahmad, A., & Sastry, M. (2003). Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnology Progress, 19, 1627–1631.
- 6) Chandran, S. P., et al. (2006). Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnology Progress, 22, 577–583.
- 7) Mukherjee, P., et al. (2001). Bioreduction of AuCl₄⁻ ions by the fungus Verticillium sp. and surface trapping of the gold nanoparticles formed. Angewandte Chemie International Edition, 40(19), 3585–3588.
- 8) Ahmad, A., et al. (2003). Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids and Surfaces B: Biointerfaces, 28, 313–318.
- 9) Vigneshwaran, N., et al. (2006). A novel one-pot green synthesis of stable silver nanoparticles using soluble starch. Carbohydrate Research, 341(12), 2012–2018.
- 10) Shankar, S. S., et al. (2004). Rapid synthesis of Au, Ag, and bimetallic Au core–Ag shell nanoparticles using neem (Azadirachta indica) leaf broth. Journal of Colloid and Interface Science, 275(2), 496–502.
- 11) Edwards-Jones, V. (2006). Antimicrobial and barrier effects of silver against methicillin-resistant Staphylococcus aureus. Journal of Wound Care, 15, 285–290.
- 12) Bindhani, B. K., & Panigrahi, A. K. (2014). Green synthesis of gold nanoparticles using neem (Azadirachta indica L.) leaf extract and its biomedical applications. International Journal of Biomedical Research, 15(3), 457–464.
- 13) Ahmed, S., et al. (2015). Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. Journal of Radiation Research and Applied Sciences, 9(1), 1–7.
- 14) Anitha Kumari, S., Patlolla, A. K., & Madhusudhanachary, P. (2022). Biosynthesis of silver nanoparticles using Azadirachta indica and their antioxidant and anticancer effects in cell lines. Micromachines, 13(9), 1416.
- 15) Otunola, G. A., et al. (2017). Characterization, antibacterial and antioxidant properties of silver nanoparticles synthesized from aqueous extracts of Allium sativum, Zingiber officinale, and Capsicum frutescens. Pharmacognosy Magazine, 13, S201–S208.
- 16) Adebayo-Tayo, B., Salaam, A., & Ajibade, A. (2019). Green synthesis of silver nanoparticles using Oscillatoria sp. extract and evaluation of antibacterial, antibiofilm, and cytotoxic activities. Heliyon, 5(10), e02502.
- 17) Singh, D. A., et al. (2014). Synthesis and characterization of sol–gel prepared silver nanoparticles. International Journal of Nanoscience, 13(1), 1450004.
- 18) Ahmed, S., Ahmad, M. S., & Swami, B. L. (2016). Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. Journal of Radiation Research and Applied Sciences, 9(1), 1–7.
Explore Our Related Journals
Looking for the right journal for your next manuscript? Explore our international peer-reviewed journals covering engineering, management, computer science, artificial intelligence and multidisciplinary research.